![]() Ticks (Acari: Ixodoidea) represent a major threat to human and animal health worldwide due to their major role as vectors and reservoirs of a variety of zoonotic protozoan, bacterial and viral pathogens. ![]() This information adds to the knowledge of TBPs epidemiology and will contribute to the scientific basis for planning future control strategies. burnetii in ticks, considering its pathogenic potential, it is essential to continue monitoring for its possible recurrence in ticks. parva p104 genes were conserved among pathogens in the four districts. The MIR of tick-borne pathogens (TBPs) differed significantly among districts, seasons, tick species, and tick developmental stages. burnetii was never detected in any tick DNA pool. However, PCR screening of individual tick DNA revealed that only 0.3% of the examined pools had co-infection. Multiple pathogens detection was observed in 7.2% of the tested pools. Theileria parva was the most abundant with a minimum infection rate (MIR) of 2.8%, followed by B. Out of 377 pools, 34.7% were positive for at least one pathogen. DNA from ticks was pooled according to tick species, developmental stage, and location, then screened for Babesia bigemina, Babesia bovis, Theileria parva and Coxiella burnetii. In total, 2021 ticks comprising nine species were identified. We used nested PCR and sequencing to screen pathogens of public and veterinary health importance in ticks collected by flagging from four districts of Tanzania. Despite their wide distribution, information on the tick and pathogen relationship is scarce in Tanzania. ![]() TBDs pose serious constraints to the improvement of livestock production in tropical and subtropical regions of the world. Ticks are vectors and reservoirs of a variety of pathogens including protozoa, bacteria and viruses which cause tick-borne diseases (TBDs) in humans and livestock. ![]()
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